Journal: IBRO Neuroscience Reports

Article Title: TNKS1 mediates the PTEN-PI3K/AKT pathway to regulate glycolysis and proliferation in gliomas

doi: 10.1016/j.ibneur.2026.01.007

Figure Lengend Snippet: TNKS1 can activate the PI3K/AKT pathway by inhibiting the expression of PTEN in glioma cells.Western blotting was used to detect the expression of PTEN in U87 and U251 cell lines (A); the CCK-8 assay was used to measure the viability of U87 and U251 glioma cells treated with different concentrations of LY294002 and 740 Y-P (B); Western blotting was also used to detect the expression of PI3K, p-AKT/AKT in U87 and U251 cell lines (C).*P < 0.05 vs si-NC，@P < 0.05 vs si-NC+Agonist，#P < 0.05 vs si-TNKS1，&P < 0.05 vs OE-TNKS1.

Article Snippet: The cells were then cultured in the incubator for another 12 h. Subsequently, the cells in each well were randomly divided into groups: the si-NC+agonist group, the si-TNKS1 +agonist group, and the OE-TNKS1 +inhibitor group were treated with PI3K agonist 740 Y-P (HY-P0175, MCE, USA) and PI3K inhibitor LY294002 (HY-10108, MCE, USA) and continued to be cultured for 24 h. The TNKS1-siRNA empty vector group (si-NC), the TNKS1-siRNA group (si-TNKS1), and the TNKS1 overexpression group (OE-TNKS1) were treated with an equivalent amount of diluent and continued to be cultured for 24 h.

Techniques: Expressing, Western Blot, CCK-8 Assay

Journal: IBRO Neuroscience Reports

Article Title: TNKS1 mediates the PTEN-PI3K/AKT pathway to regulate glycolysis and proliferation in gliomas

doi: 10.1016/j.ibneur.2026.01.007

Figure Lengend Snippet: TNKS1 can regulate the proliferation and glycolysis of glioma cells by mediating the PTEN-PI3K/AKT pathway.Cell viability of various groups in U87 and U251 cell lines (A); detection of glucose (GLU) and lactate (LA) levels in the culture supernatant of various groups in U87 and U251 cell lines using biochemical assay kits (B); qPCR was used to detect the mRNA levels of GLUT1 and HK2 in various groups of U87 and U251 cell lines (C). Statistical significance *p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001,"ns" P ≥ 0.05.

Article Snippet: The cells were then cultured in the incubator for another 12 h. Subsequently, the cells in each well were randomly divided into groups: the si-NC+agonist group, the si-TNKS1 +agonist group, and the OE-TNKS1 +inhibitor group were treated with PI3K agonist 740 Y-P (HY-P0175, MCE, USA) and PI3K inhibitor LY294002 (HY-10108, MCE, USA) and continued to be cultured for 24 h. The TNKS1-siRNA empty vector group (si-NC), the TNKS1-siRNA group (si-TNKS1), and the TNKS1 overexpression group (OE-TNKS1) were treated with an equivalent amount of diluent and continued to be cultured for 24 h.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: TNKS1 mediates the PTEN-PI3K/AKT pathway to regulate glycolysis and proliferation in gliomas

doi: 10.1016/j.ibneur.2026.01.007

Figure Lengend Snippet: TNKS1 can regulate the PTEN-PI3K/AKT pathway in glioma xenografts.Western blotting was used to detect the protein expression levels of PTEN, PI3K, p-AKT, and AKT in cells from each group.* P < 0.05 vs TNKS1-siRNA NC, @ P < 0.05 vs TNKS1-siRNA，# P < 0.05 vs Inhibitor.

Article Snippet: The cells were then cultured in the incubator for another 12 h. Subsequently, the cells in each well were randomly divided into groups: the si-NC+agonist group, the si-TNKS1 +agonist group, and the OE-TNKS1 +inhibitor group were treated with PI3K agonist 740 Y-P (HY-P0175, MCE, USA) and PI3K inhibitor LY294002 (HY-10108, MCE, USA) and continued to be cultured for 24 h. The TNKS1-siRNA empty vector group (si-NC), the TNKS1-siRNA group (si-TNKS1), and the TNKS1 overexpression group (OE-TNKS1) were treated with an equivalent amount of diluent and continued to be cultured for 24 h.

Techniques: Western Blot, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Paeonol inhibits the development of oral squamous cell carcinoma through the PI3K/AKT signaling pathway

doi: 10.3389/fcell.2026.1747424

Figure Lengend Snippet: Pae induces apoptosis in OSCC cells via suppression of PI3K/AKT signaling. (A) FC analysis of apoptosis in CAL-27 and HSC-3 cells treated with Pae. (B) WB quantification of apoptosis-related proteins (Bax, Bcl-2, caspase-3 forms). (C) WB evaluation of PI3K/AKT phosphorylation post-Pae treatment. (D) Protein expression analysis following combined Pae and PI3K activator treatment.

Article Snippet: In addition, MedChemExpress (USA) supplied the cell-permeable PI3K activator 740Y-P (HY-P0175).

Techniques: Phospho-proteomics, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Paeonol inhibits the development of oral squamous cell carcinoma through the PI3K/AKT signaling pathway

doi: 10.3389/fcell.2026.1747424

Figure Lengend Snippet: The autophagy inhibitor 3-MA potentiates Pae-induced antiproliferative and apoptotic responses in OSCC cells. (A) WB and quantification of LC3 and p62 expression in OSCC cells pretreated with 3-MA (2.5 mM, 1 h) followed by Pae (1.6 mM, 24 h). (B) WB analysis and densitometric quantification of phosphorylated and total PI3K and AKT protein expression post-treatment. (C) Expression levels of apoptosis-related proteins Bcl-2 and Bax after combined treatment.

Article Snippet: In addition, MedChemExpress (USA) supplied the cell-permeable PI3K activator 740Y-P (HY-P0175).

Techniques: Expressing

Journal: Endocrine Connections

Article Title: Effects of miR-451 on the proliferation, invasion and migration of papillary thyroid cancer cell line TPC-1 by regulating the PI3K/AKT/mTOR signaling pathway

doi: 10.1530/EC-25-0802

Figure Lengend Snippet: High expression of miR-451 inhibits the activation of PI3K/AKT/mTOR signaling pathway. (A) Proteins expression levels interrelated with the PI3K/AKT/mTOR signaling pathway in TCP-1 cells; (B) bar chart of proteins interrelated with the PI3K/AKT/mTOR signaling pathway in TCP-1 cells; ** P < 0.001.

Article Snippet: The PI3K activator 740Y-P was purchased from MedChemExpress (USA).

Techniques: Expressing, Activation Assay